Thursday 12 January 2017

Trypticase Soy Agar (TSA) Plate

  • ·         Casein Enzymatic Hydrolysate  (Tryptone) ---------------------------15.00g
  • ·         Papic Digest of Soya been meal (Soy peptone) or (peptone S2)--- 5.00g
  • ·         Sodium Chloride ------------------------------------------------------------ 5.00g
  • ·         Lecthitin-----------------------------------------------------------------------0.70g
  • ·         Poly sorbate 80 or Tween 80-----------------------------------------5.00g
  • ·         Agar-----------------------------------------------------------------------15.00g
  • ·         Glycerol------------------------------------------------------------------  1.00%

 Ø  Betalactamase---------------------------------------------4.5 IU/plate
Ø  Each plate 30 medium
Ø  Bacterial growth purose -------------------------------- 5 % v/v sheep/ horse blood used.


With Betalactamase------------- inactivation of the penicillin, Cephalosporin 

  • ·         Triple Wrap and Barrier Wrap packaging for Cleanroom or Isolator environments.
  • ·         Triple bag products provide 3 layers of protection and sterile storage of unopened plates.
  • ·         Protective layers enhance moisture control by incorporating novel elements that permit the passage and absorption of excess moisture, reducing the risk of contamination.
  •             BAM (Bacteriological Analytical Manual)media M152.


What is the fumigation and fogging?

What is the fumigation and fogging?