How to ensure autoclave effectiveness ?

• Autoclaves mainly used for sterilization process.
• Autoclave Sterilization temperature at 121⁰C for 15 to 20 minutes depending on the size of the load and the contents.
• Sterilization in an autoclave is most effective when the organisms are either contacted by the steam directly.
• Under these conditions, steam at a pressure about  15 PSI. attaining temperature (121⁰C) will kill all organisms.
• The autoclave was invented by  Charles Chamberland in 1879.
•  This process ensure the 3 parameters

Related: Classification of Chemical Indicators

           1.       Physical  : Pressure, temperature and time recording devices.

           2.       Chemical :  Indicators that change color after being exposed to specific temperatures, such as              temperature sensitive tape.

           3.       Biological : Geo bacillus stereo  thermophilus  spores are used and due to its resistance to                    heat, for the testing that measures the biological performance of the autoclave process.

        Related : Types of Biological Indicators