- Chemical formula Nacl.
- 1:1 ratio of Sodium and chloride ions.
- Is also called in salt solution or saline solution.
Water insoluble matter -------------0.003%
Bromide & Iodide --------------------0.005%
Ferro cyanide------------------------0.0001%
Phosphate (Po4) ------------------0.0005%
Sulphate (So4) -------------------------0.002%
Total nitrogen ---------------0.001%
Arsenic (As) ---------------------------0.00004%
Barrium (Ba) ---------------------0.001%
Calcium (Ca) -------------------0.002%
Copper (Cu) ----------------------0.0002%
Iron (Fe) ------------------------0.0002%
Lead (Pb) ------------------------0.0002%
Potassium (K) ------------------0.01%
Magnesium (mg) -------------0.002%
pH Range -------------- 5.0 to 8.0%
- Sterile 0.9% saline is called normal saline and isotonic saline.
- It is sodium chloride.
- 9 grams of Nacl weighed and dissolve the one liter of purified water.
- Sodium chloride main role is avoid the dehydration.
- Sterile saline solution is used for the preparation of culture suspension or serial dilution in microbiology.
- Culture suspension and serial dilution are prepared in sterile saline solution because 0.9% sodium chloride solution is isotonic in nature.
- In isotonic solution the concentration of solutes remains the same both inside and out side of the microbial cell and cells remains at their usual osmotic pressure.
- But water is hypo tonic in nature in which the concentration of solute is greater inside the cell than outside and due this water try to enter inside the bacterial cell. the osmotic pressure develop on the bacterial cell which can damage the bacterial cells during serial dilution. That's why serial dilution is prepared in 0.9% saline solution.
