- Is called most probability number or microscope method.
- 2 spores can grow on one cfu.
- Microorganisms are merged at an impaction site to a produce a single colony.
- Sampling unit collected cells multiply in to colonies.
- Quantitative data
- Many discrete entries easily detected but difficult to count.
- Viable particles to the petriplate and conversion to this count to a particle count by use of positive hole conversion.
Role:
- Number of the viable particles being impinged on probability of the next particle going into an "empty hole" decreases
= 380 (1/380 + 1/(380-1) + 1/(380-2) +
1/(380-2+1)
= 380 X 0.00263 + 0.00263 + 0.00264---------------+ 0.00265
= 380 X 0.01055
=4.009
1---------------3
2---------------4
