·
Culture
media contains nutrients and physical growth parameters necessary for
microbial growth. All microorganisms cannot grow in a single culture medium and
in fact many can’t grow in any known culture medium.
·
Organisms
that cannot grow in artificial culture medium are known as obligate
parasites. Mycobacterium and Chlamydias are obligate parasites. Bacterial culture media can be
distinguished on the basis of composition, consistency and purpose.
1.
Solid medium (Agar medium):
solid medium contains agar at a concentration of 1.5-2.0% or some other, mostly inert solidifying agent. Solid medium has physical structure and allows bacteria to grow in physically informative or useful ways (e.g. as colonies or in streaks). Solid medium is useful for isolating bacteria or for determining the colony characteristics of the isolate.
solid medium contains agar at a concentration of 1.5-2.0% or some other, mostly inert solidifying agent. Solid medium has physical structure and allows bacteria to grow in physically informative or useful ways (e.g. as colonies or in streaks). Solid medium is useful for isolating bacteria or for determining the colony characteristics of the isolate.
2.
Semisolid media:
They are prepared with agar at concentrations of 0.5% or less. They have soft custard like consistency and are useful for the cultivation of microaerophilic bacteria or for determination of bacterial motility.
They are prepared with agar at concentrations of 0.5% or less. They have soft custard like consistency and are useful for the cultivation of microaerophilic bacteria or for determination of bacterial motility.
3.
Liquid medium(Broth medium):
These media contains specific amounts of nutrients but don’t have trace of gelling agents such as gelatin or agar. Broth medium serves various purposes such as propagation of large number of organisms, fermentation studies, and various other tests. Example : SCDM & RVSEB.
These media contains specific amounts of nutrients but don’t have trace of gelling agents such as gelatin or agar. Broth medium serves various purposes such as propagation of large number of organisms, fermentation studies, and various other tests. Example : SCDM & RVSEB.
Types of
media:
1.
Complex
media
2.
Defined
media
3.
differential
media
4.
Enrichment
media
5.
Enriched
Media
6.
Thermolaible
media
7.
Selective
media
8.
Basel
media
9.
Storage
media
Culture:
The microbes can grow and Multiply. In or an
culture medium.
Culture Medium:
This nutrient
medium Prepared for a growth of microorganism in laboratory.
Nutrient Broth:
If the
Complex media in liquid form.
Nutrient agar:
If the complex media in solid Forms, agar is
added.
Selective Media:
Suppression of unwanted microbes and encouraging the desired microbes.
Differential media (Indicative media):
Differentiation of colonies.
Enrichment Media:
Similar to
selective but designed to increasing the number of colonies of desired microbes to detectable levels.
Enrichment
media is liquid forms that inhibited the unwanted microbes.
Enriched Media:
Additional
source of blood are serum having.
Solid media
like chocolate agar media and blood agar media.
Basel Media:
Used for
growth (culture) of bacteria that do not need enrichment of the media.
Ex:
Nutrient Agar
Nutrient
Broth
Peptone
water.
Storage media:
Media used
for long time storage purpose.
Ex : Egg saline medium
Chalk cooked
meat broth.
