Cultivation of microorganisms by letting them reproduce in predetermined culture media
under controlled condition.
Media consistent:
Amino
nitrogen – peptone, protein, hydrolysate, infusions, and extracts.
Growth
Factors – Blood, serum, yeast extract and vitamins.
Energy
sources – sugar, alcohols and carbohydrates.
Mineral salts
and metals – phosphate and sulfate.
Selective
Agents – chemicals, antimicrobial and dyes (Ex: Dehydrate Oxibile, Bile salt
mixer and Malachite green).
Indicator
dyes – Phenol red, Neutral red and Bromo Cresol Purple (BCP) dye.
Gelling
agents- Agar, Silica gel, Gelatin, Alginate and Albumin.
Supplements-
Glycerol, Neledixic selective medium.
