- Mannitol salt Agar
- It is selective media for pathogenic Staphylococci. (non-motile)
- It contains high concentration of salt (Nacl) 7.5 to 10%.
- Selective gram possitive staphylococci
- Nacl is inhibitory most of the bacteria
- Staphylococcus aureus produce yellow colonies and yellow zones.(Catalase possitive )
- Staphylococcus Epidermidis produce to pink colour colonies (catalase negative)
- Note : This product contains 7.5% Sodium chloride as one of its ingredients. On repeated exposure to air and absorption moisture sodium chloride has tendency to form lumps, therefore we strongly recommend storage in tightly closed containers in dry place away from bright light .
Composition
Grms/lit
Enzyamatic digest of animal tissue
(proteose peptone) ------------10.000
Beep
extract-------------------------------------------------------------------1.000
Sodium
chloride----------------------------------------------------------75.000
D.mannitol---------------------------------------------------------------10.000
Phenol
red-------------------------------------------------------------0.025
Agar--------------------------------------------------------------------------15.000g
pH= 7.4 ± 0.2 at 250C
- Enzyamatic digest of animal tissue (proteose peptone) and beef extract –suply growth factros
- Sodium chloride---------------------inhibitory agent other bacteria.
- D-mannitol----------------------fermentable carbohydrate
- Phenol red----------colour indicator
- Agar----------------solidifying agent.
- Phenol sulfur phthalien or PSP
- Is a pH indicator.
Below 6.8 Above pH 8.2
…Yellow…… … ROSE……..
Yellow colonies /
white colonies and surrounding by yellow Zones.
