Phosphate Buffered saline

• Phosphate Buffered saline is a buffer solution.
• It is a water based salt solution.
• PBS solution used in rinsing (washing cells) in microbial analysis because it is isotonic and non-toxic to most cells.
• PBS solution used in serial dilution (diluting cells) for cell counting.  
• PBS solution used in reagent preparation.

    Composition                                     grams/liter

Sodium Chloride--------------- ---------------8.50

Di Sodium hydrogen phosphate----------1.910

Potassium di hyrogen phosphate---------0.380

          

pH= 7.2 ±0.2 at 25⁰C

• Heat if necessary to dissolve the medium completely.
• Sterilize by autoclaving 10 lbs pressure (115⁰C) for