- · BAM (Bacteriological Analytical Manual)media M127
- · Is used for cultivation of fungi (yeast and molds).
- · Potato and dextrose encourage the luxurient fungal growth.
- · Nutritionally high rich base medium.
- · Low pH medium, inhibiting the bacteria
- · Adjustment the pH medium by 10 % Sterile tataric acid (1 ml of Acid 100ml of Medium) 3.5 ±0.2
- · Do not heat the medium after addition of the acid.
- · The nutritionally rich base (potato infusion) encourages mold sporulation and pigment production in some dermatophytes.
Composition
grm/lit
Potato extract
----------------------------------4g
Dextrose (glucose)------------------------------20g
Agar-----------------------------------------------15g
pH=5.6 ±0.2 at 250C
- · 4 grms of potato extract equal to 200g of potato infusion
- · potato extract encourage the pigment production of dermatopytes(skin infections)
- · dextrose (glucose) source of energy.
- · Agar---solidifying agent.
- · Additionally Chloramphenicol acts as a selective agent to inhibit bacterial overgrowth of competing microorganisms from mixed specimens, while permitting the selective isolation of fungi.
