- · Soya Bean casein digest medium or Casein of Soya Bean digesist medium
- · Is also called Trypticase soy broth or tryptone soya broth (TSB)
- · Highly nutrient medium
- · Soyabean Casein Digest Medium is a general purpose medium.
- · Used for cultivation of a wide variety of microorganisms and recommended for sterility testing of moulds and lower bacteria.
- · They are general-purpose, nonselective media providing enough nutrients to allow for a wide variety of microorganisms to grow.
- · They are used for a wide range of applications, including culture storage, enumeration (counting), isolation of pure cultures, or simply general culture.
Composition
Gram/liters
Pancreatic digest of casein (peptone
C)--------------------------17.000
Papaic digest of soya bean meal (soy
peptone)(peptoneS2)---3.000
Dextrose
(glucose)---------------------------------------------------2.500
Sodium chloride-------------------------------------------------------5.000
Di potasium
phosphate---------------------------------------------2.500
pH = 7.3±0.2 at 250C
Appearance :
Cream to yellow
- · Pancreatic digest of casein (peptone C or Casein peptone) and papaic digest of soya bean meal (soy peptone or peptone S2) source of amino acid grow of microorganism.
- · Dextrose (glucose) source of carbon
- · Sodium chloride-------osmotic balance
- · Di potassium phosphate------pH balance (buffer)
Note: If any fibers are observed in the solution,it is
recommended to filter the solution by using a 0.22 micron filter to eliminate
the possibility of presence of fibers.
·
TSA
contains enzymatic digests of casein in place of Pancreatic digest of
casein.
