- The purpose of the negative control is to show that there is no contamination during the testing of the product.
- Negative control verify the testing environment, testing condition, analyst handling and glassware -----etc. which were used during the testing.
- Negative control must be included during every testing like product testing, water testing, environmental monitoring……etc. this is the pharmacopoeial requirement.
- Negative control is performed using the chosen diluent in place of the test preparation. There must be no growth of microorganisms.
- A failed negative control requires an investigation.
- If a positive results is obtained with negative control , the test can be regarded as invalid and may be repeated.
- According to pharmacopoeia if there is any product failure no repeat testing is allowed unless otherwise it has been confirmed during investigation that test is invalid.
- That’s why negative controls are very important and should be used during every testing.
- Negative control should be incubated till the completion of the incubation of particular test.
