- Any specified identification techniques of the In-house isolates and their library will help pharmaceutical to follow the proper guidelines.
- Mainly three method of identification process.
1. Phenotypic identification
2. Proteotypic identification
3. Genotypic identification
- The genetypic method is considered to be more accurate than the phenotype which useful for sterility testing.
- Genotypic identification uses the DNA sequences where are phenotype identification involves describes the protein or peptide sequence.
- Accurate classification of un known bacterial isolates is an essential first step in understanding the impact these organisms have on an environmental monitoring programme.
- They are many methods, technologies and strategies utilized to determine the identity of unknown microorganisms, however the selection of these methods is often impacted by more than performance of technology.
- Cost, time and amount is impact the identification process.
1. Proteotypic identification : Bruker Bio Typen MALDI-TOF spectroscopy.
2. Metabolic Profiling : Biomerieux -VITEK 2 Compact
This equipment given the results based up on biochemical tests.
3. DNA sequence analysis:
