- Violet Red Bile Glucose Agar or Violet Red Bile Dextrose Agar.
- Do not Autoclave and Do not Overheat.
- Selective media for Enterobacterium (Bile tolerant g-ve Bacteria).
- Enterobactriaceae includes lactose-fermenting colifrom bacteria, non-lactose fermenting of Escherichia coli.
Composition
Gram/liter
Peptic digest of Animal
tissue----------------------7.00g
Yeast
extract--------------------------------------------3.00g
Sodium
Chloride---------------------------------------5.00g
Bile salt
mixer-----------------------------------------1.50g
Glucose (Dextrose)---------------------------------10.00g
Neutral
red-----------------------------------------0.030g
Crystal
violet---------------------------------------0.002g
Agar-------------------------------------------------
12.00g
pH- 7.4 ± 0.2 at 250C
- Peptic digest of Animal tissue and Yeast extract source of carbon, nitrogen,essential minerals and B- complex vitamins to stimulate the growth of bacteria.
- Glucose (Dextrose) supplies energy for growth and metabolisms.
- Sodium Chloride source of osmotic balance.
- Bile salt mixer and Crystal violet inhibit the growth of gram positive bacteria.
- Neutral red is added as a color and pH indicator.
- Agar is the solidifying agent.
- Organisms that ferment glucose will produce red to purple colonies with red- purple halos, demonstrating bile precipitation in the presence of neutral red.
