- Xylose- Lysine Deoxy cholate Agar
- XLDA formulated by Taylor.
- Selective medium salmonella typi and other salmonell spp.
- Sodium Deoxy cholate is the selective agent.
- Do not Autoclave or over heating.
- Heat with agitation and pour the plate.
Ingredients
Gram / Liter
Yeast extract
------------------------------------------------------3.000
L-Lysine-----------------------------------------------------------
5.000
Lactose --------------------------------------------------------------7.500
Sucrose
--------------------------------------------------------------7.500
Xylose
---------------------------------------------------------------3.500
Sodium chloride ----------------------------------------------------5.000
Sodium
deoxycholate----------------------------------------------- 2.500
Sodium
thiosulphate-------------------------------------------------- 6.800
Ferric ammonium
citrate-------------------------------------------- 0.800
Phenol red-------------------------------------------------------------- 0.080
Agar
---------------------------------------------------------------------15.000
- Yeast extract ---------nitrogen and vitamins (growth medium)
- Sugars (xylose, lactose, sucrose) provided carbohydrate fermentation.
- L-lysine mainly incorporate the Shigella spps.
- Sodium Chloride ------------osmotic balance
- Sodium deoxycholate-------------inhibit the gram positive organism.
- Sodium thiosulphate and Ferric ammonium citrate ---------hydrogen sulphide (H2S) produced visvalization of colonies formation of with black centers.
- Phenol red ----------pH indicator
Note: Slight
precipitation in the medium may occur, which is inherit ate properties of the
medium and does not affect the performance of the medium.
- Salmonella spps ---------------red colonies with black centers is produced.
- Psedomonas aeruginosa --------------red colonies with out black centers is produced (flase positive result).
- E.coli ------------------------------Yellow colonies
- Shigella------------------------Red colonies.
