Fluid Thioglycollate Medium (FTGM)

• FDA BAM (Bacteriological Analytical Manual) media M146.
• Is used for sterility testing of biologicals and for cultivation of anaerobes, aerobes and microaerophiles..
• Falk, Bucca and simmons was discovered.
• Used for cultivation of anaerobic bacteria and other bacteria.

Composition                                              Gram/liter

Pancreyatic digest of casein -------------------15g

Yeast extract---------------------------------------5g

Dextrose (glucose)------------------------------5.5g

L-crystine-----------------------------------------0.5g

Sodium chloride---------------------------------2.5g

Sodium thioglycollate--------------------------0.5g

Resazurin sodium ----------------------------0.001g

Agar------------------------------------------------0.75g

pH = 7.1 ± 0.2 at 25⁰C

• Pancreyatic digest of casein and yeast extract source of nitrogen vitamins and carbon.
• Sodium Thoglycollate and L-crystine --- reduction of oxidation (removing of oxigen)
• Resazurin sodium --------------oxidasation  indicator  (in the state of oxygen reszurin show pink)
•  Dextrose (glucose)----------enhance the organism growth.
•  Sodium chloride----------maintained the osmotic balance.
•  Agar----------- co2 dispersion and diffusion of oxygen and reducing substances.

• Agar helps maintaining low redox potential for stabilizing the medium.(Redox = oxygen reduction)

Note 1 : if more than the upper one-third of the medium has acquired a pink colour, the medium may be restored by heating in a water bath. Or free –flowing steam until pink colour disappears.

Note 2:  This medium first light yellow colour and pink colour ring apper, after storage showing dark yellow colour and pink colour  ring apper.

• Sodium Thioglycollate – Neutralizing the Mercurials.