0.9% Saline solution is
the best dilution for cell count.
Saline water isotonic solution (no swelling or shrinking of
cell) same as cell protoplasm, minimizes the chances of cell death.
Phosphate buffer saline (PBS) solution also suitable for
serial dilution preparation.
Buffered sodium chloride peptone (BSCP) solution or peptone
solution is not suitable for serial dilution because it supports growth and
after utilizing the nutrients (peptone) bacterial cell die.
Saline is 0.9% sodium chloride(Nacl) solution.
Serial dilution or culture suspension used saline solution
(0.9% Nacl) because 0.9% sodium chloride (Nacl) solution is isotonic in nature.
In isotonic solution the concentration of solutes remains
the same both inside and outside of the microbial cell and cells remains at
their usual osmotic pressure.
But water is hypo tonic in nature in nature in which the
concentration of solute is grater inside the cell than outside and due to this
water try to enter inside the bacterial cell.
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