- · FDA BAM (Bacteriological Analytical Manual)media M133
- · It is containing of peptone.
- · Cultivation of dermatophytes (skin infection)and other type of fungi and also grow filamentous bacteria such as “Nocardia”.
- · It was created by Raymond sabouraud.
- · Lower pH to support the fungi (acidic pH) inhibit the bacteria.
- · Sabouraud Dextrose Broth (SDB) is a selective enrichment medium primarily used for the isolation of dermatophytes, other fungi and yeasts but can also grow filamentous bacteria such as Nocardia.
- · Antibacterial (Antibiotics) agents can also be added to augment the antibacterial effect.
- · Antibiotics like chloramphenicol, gentamicin, and tetracycline can be added as selective agents to inhibit bacterial growth.
Composition Grams/liter
Enzymatic digest of casien ---------------5g peptone= 10g
Enzymatic digits of animal tissue---------5g
Dextrose (glucose)----------------------------40g
pH= 5.6 ±0.2 at 250C
·
Enzymatic digest of casien and Enzymatic
digits of animal tissue (Peptone) provide
a nutritious source of amino acids and nitrogenous compounds for the growth of
fungi and yeasts.
·
Dextrose (glucose) source of energy.
·
Addition of antibiotics like Chloramphenicol and/or
tetracycline acts as broad spectrum antimicrobials to inhibit the growth of a
wide range of gram-positive and gram-negative bacteria.