- There is no strict definition of this group of micro-organisms. They are defined operationally as those micro-organisms that show growth in the stated conditions on violet red bile glucose agar (VRBGA) medium.
- They include, gram negative bacteria that grow in the presence of bile salts, non-lactose fermenting but also to utilize glucose.
- Some bile tolerant gram negative bacteria includes members of the family Enterobacteriaceae, Pseudomonas and Aeromonas.