Sabouraud Dextrose Agar (SDA)

·                ·         It is containing of peptone. ( BAM (Bacteriological Analytical Manual)media M133.
          ·         Cultivation of dermatophytes (skin infection)and other type of fungi and also grow filamentous bacteria such as “Nocardia”.

• ·         It was created by Raymond sabouraud.
• ·         Lower pH to support the fungi (acidic pH) inhibit the bacteria.
• ·         Sabouraud Dextrose Agar  (SDA) is a selective medium primarily used for the isolation of dermatophytes, other fungi and yeasts but can also grow filamentous bacteria such as  Nocardia.
• ·         Antibacterial (Antibiotics) agents can also be added to augment the antibacterial effect.
• ·         Antibiotics like chloramphenicol, gentamicin, and tetracycline can be added as selective agents to inhibit bacterial growth.

Composition                                                          grms/lit

Enzymatic digest of casien ---------------5g                   

Enzymatic digits of animal tissue---------5g

Dextrose (glucose)----------------------------40g

Agar---------------------------------------------15g

• Enzymatic digest of casien and Enzymatic digits of animal tissue called peptone)
• Total peptone 10 g.

pH= 5.6 ±0.2 at 25⁰C

·         Enzymatic digest of casien and Enzymatic digits of animal tissue (Peptone)  provide a nutritious source of amino acids and nitrogenous compounds for the growth of fungi and yeasts.

·         Dextrose (glucose) source of energy.

·         Agar source of solidifying agent.

·         Addition of antibiotics like Chloramphenicol and/or tetracycline acts as broad spectrum antimicrobials to inhibit the growth of a wide range of gram-positive and gram-negative bacteria.

Example :

    Ø        Canidida Albicans  (Yeast)– White colour colonies observed.

    Ø        Aspergillus Brsilensis (mold) –Black colour molud.