- Selective enrichment medium.
- Selective Gram negative E.Coli
Comoposition Grams/liter
Pancratic digesit of gelatin
-----------------------------20.000
Lactose monohydrate--------------------------------------10.000
Dehydrate Oxi
Bile-------------------------------------------5.000
Bromo Cresol Purple (BCP) dye--------------------------0.01
pH = 7.3 ±
0.2 at 250C
- Pancratic digist of gelatin -----produce a nutrient
- Lactose monohydrate--------------------------fermantable carbohydrate
- Lactose fermenting organisms turn the medium yellow due to the acidity produced on lactose fermentation.
- Dehydroxy oxi bile ------------------inhibit the gram possitive organisum
- BCP (Bromo cresol purple) dye ----------- is the pH indicator in the medium which turn to yellow under acidic condition.
BCP:
- Bromo Cresol purple Dye
- Prepared as a 0.04% aqueous solution.
- Primary function indicator.
- It is used to measure albumin.
- pH above 6.8 -purple colour …………..
- pH below 5.2 -yellow colour …………..
